Chromatographic analysis of decoys extracts used for control of haylomma dromedary ticks of camels

ABSTRACT

Chromatographic [thin layer chromatography, TLC and high pressure liquid chromatography, HPLC] analysis of cholesteryl esters of decoys prepared from female ticks of camels [Haylomma dromedarii] showed cholesteryl acetate, cholesteryl laurate and cholesteryl oleate, in concentration of 0.88053, 4.7433 and 2.5081 ug/5 decoys, respectively. These values decreased by time of attachment of the decoy to rabbits infested with male ticks till it became 0.6509, 2.0120 and 0 ug/5 decoys, respectively, after 20 days of attachment. Cholesteryl oleate disappeared from the decoy after 10 days of attachment. Mixing of cyfluthrin with 2,6-dichlorophenol [2,6-DCP] to the composition of decoys, their concentration decreased from time of attachment of them till they became 1.01231 and 0.004826 ug/5 decoys after 20 days. In vitro studies showed that addition of cyfluthrin to engorged female Haylomma dromedarii ticks in concentrations of 0.15 and 0.075/100 ml water, respectively, died within 30 days while their keeping in cyfluthrin in concentration of 0.6 ml/100 ml water caused their death within 7 days without egg production