new more simpler and rapid technique for extracting a genomic DNA from mycobacterial strains

ABSTRACT

A rapid simple method for extraction of the DNA from mycobacteria is described. The extraction of DNA from mycobacteria, simply depends upon the lysis of cell wall, using sodium hydroxide, sodium dodecyl sulfate and sodium chloride. The resulting DNA has been checked by other tests such as polymerase chain reaction [PCR] and the results indicated that the extracted DNA works well with these tests