Direct and via callus root regeneration in atropa acuminata royle

ABSTRACT

Direct rooting was induced in 4 weeks from midrib of Atropa acuminata leaves with 0.1 mg/1 2, 4-D. Same treatment was found best also for callus formation. Callus growth was carried over more than a year with K + AS at different concentrations. Active root regeneration via callus was induced in old cultures with 1 mg/1 AS. Root regeneration was successful in 62.5% cultures