new modified method for the detection of antibody producing cells during parasitic infections

ABSTRACT

A modified method for detecting antibody producing cells [APC] was described in this work The method depends on spreading the cell mixture prepared for the assay in a single layer on a gelatin cusion without using a solidifying medium. The method was proved to be highly sensitive and less costly. The method was applied to detect APC during infection with H nana. Infected animals showed fewer APC in their splenocytes when compared with a control group