Effect of anoxia and heat on the ascorbic acid content and phosphomonoesterases actives of mouse adrenal cortex

ABSTRACT

9 male mice were included in the present study. They were kept in individual small cages in an oven of constant temperature at 45C with low oxygen content, then the adrenal glands were taken at periods of time [5 minutes, 10 minutes and 15 minutes]. The ascorbic acid content and the activities of phosphomonoesterases have been studied. The results obtained in the present study showed that, ascorbic acid was sensitive to heat and anoxia, since 5 minutes after exposure, a decrease of positive granules was observed from the superficial zones of the cortex to be concentrated in the inner zones, which showed a transport of these granules from the cells through the sinusoids. Alkaline phosphatase activity did not show any change all over this study. Initial increase in acid phosphatase after 5 minutes of exposure to heat and anoxia followed by gradual decrease in enzyme activity