effect of near UV-irradiation on hemoglobin

ABSTRACT

Oxyhemoglobin was irradiated by near UV. The time of exposure was 15-150 minutes, which corresponds to doses. It is known that near UV is absorbed by protein and heme parts of hemoglobin. The changes in buffer capacity, absorption spectrum thermodynamic parameters, conductivity, viscosity and refractive index before and after irradiation were studied. The results indicated that, changes in electronic state of HbO2, changes in spin state constant of heme iron and increase in methemoglobin band at 630 nm demonstrate different unfolding degrees. The increase in delta H degree and delta S degree are attributed to hydrogen bond degradation. The changes in Hb conductivity after irradiation gives an evidence about the appearance of hydrophobic groups. Irradiation demonstrated the unfolding and aggregation of the protein part