Evaluation of different media used for recovery of campylobacter spp

ABSTRACT

The capacity of five Campylobacter selective plating media [Campylobacter blood free, Preston, Blood agar plus Blasser-Wang supp., Brucella agar plus Blasser-Wang supp. and Columbia agar plus Blasser-Wang supp.] and 3 selective enrichment broth [brain heart infusion broth either supplemented with Preston or Blasser-Wang or cefoperazone] were tested for their sensitivity to recover the different Campylobacter biotypes [C. jejune and C. fetus] from artificially contaminated milk. Columbia agar with Blasser-Wang supplement proved to be the best selective medium for isolation of Campylobacters. While Campylobacter blood free was the poorest medium. The confirmed technique of enriched milk samples in BHI broth plus cefoperazone [32 mg/L] and its incubation microaerobically at 42C for 2 days, followed by plating on Columbia agar selective basal medium with Blasser-Wang supplement and incubated for 2 days at 42C proved to be more productive for recovery of Campylobacters from contaminated materials