Comparative analysis of ascaridial parasitic crude antigen by SDS-page and UV absorbance techniques

ABSTRACT

Ascaridial nematodes are among the well-known parasites infesting fish, birds and mammals. Crude antigen was extracted from Amplicaecum larvae [AL3], Ascaridia galli [adult worm, AG] and Neoascaris vitulorum [adult worm, AV]. The characterization of these parasitic nematodes revealed relative homologous polypeptide chains [MW 25.5 kDa] common to the three parasitic species, whereas the UV absorbance scan mode wave length [WL 200/550 nm] as related to the absorbance [AB] value 0 - 2 recorded an indicative frequency curve. The protein concentration in Amplicaecum, Ascaridia and Neoascaris figured up to 5.47 g%, 4.93 g% and 2.2 g%, respectively