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Effect of hormone sequential supplement on plant regeneration by somatic embryogenesis from hypocotyl cultures of foeniculum vularge
Alexandria Journal of Pharmaceutical Sciences. 1995; 9 (3): 179-181
in English | IMEMR | ID: emr-36214
ABSTRACT
Callus was obtained from seedling hypocotyls on an agar solidified Murashige revised tobacco [MS] medium containing 1 mg/L. 2,4-dichlorophenoxyacetic acid [2,4-D 1]. Suspension cultures were initiated from callus using a series of the following liquid media 2,4-dichlorophenoxyacetic acid 0.5 mg/L [2,4-D 0.5], 2,4- dichlorophenoxyacetic acid 0 mg/L [2,4-D 0], benzyladenine 0.5 mg/L [BA 0.5], benzyladenine 1 mg/L [BA 1] and benzyladenine 5 mg/L [BA 5]. Compact cells of meristematic nodules were obtained at periphery of callus aggregates that developed into globular, heart and torpedo stages of somatic embryos. Plant regeneration has been achieved by culturing the embryos on solid MS medium deprived of growth regulators
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Index: IMEMR (Eastern Mediterranean) Main subject: Pharmacology / Seeds Language: English Journal: Alex. J. Pharm. Sci. Year: 1995

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Index: IMEMR (Eastern Mediterranean) Main subject: Pharmacology / Seeds Language: English Journal: Alex. J. Pharm. Sci. Year: 1995