Genesis of granulation and scar tissue in otitis media: a temporal bone study

ABSTRACT

Sixty human temporal bones with evidence of middle ear exudates, from the temporal bone collection of the University of Minnesota, USA are included in this study. Temporal bones has been removed at autopsy, routinely fixed, decalcified dehydrated, embedded in celloidin, and sectioned every 20 microns. Each tenth section had been stained with hematoxylin and eosin and prepared for light microscopy. Results showed that sequential ordering of morphopathologic events, has helped to develop insight regarding the pathogenesis of granulation tissue. Four man varieties of fibrous/granulation tissue, are recognized and described. It was concluded that the pattern of genesis of the fibrocystic tissue is skeletonized. The study presented histologic documentation that the fibrocystic granulation tissue arise in the lumen from altering intraluminal contents