Activity of some diagnostic enzymes in bilharzial bladder and colonic malignant tissues

ABSTRACT

Glucose 6-phosphate dehydrogenase [G-6-PD] and pyruvate kinase [PK] were determined in 11 malignant bladder lesions, 8 tissues with bilharzial infection origin, as well as 11 unaffected [healthy] tissues. Similarly, the two enzymes were evaluated in 10 malignant and 8 bilharzial colonic tissues, compared with 8 healthy unaffected tissues. The pyruvate kinase mean activity was decreased in malignant bladder carcinoma tissues compared to unaffected ones taken from the same patients. While, G-6-PD activity was increased in malignant bladder tissues compared to healthy [unaffected] tissues, and these differences were statistically significant. No significant difference relative to normal tissues was recorded in the activity of the two enzymes as assayed in the isolated colonic malignant tissues. It is evident that bilharzial infection causes a significant inhibition in the activities of both enzymes in bladder tissues compared to malignant tissues, while colonic bilharzial lesions had significantly lower PK and G-6-PD activity. This inhibition become statistically insignificant in colonic tissues comparing to unaffected ones. It was concluded that both enzymes are valuable biomarkers in prognosis of malignant tumors together with their diagnostic role in bilharzial malignant tumors