Serodiagnosis of extrapulmonary mycobacteriosis by ELISA using 30,000 Da and PPD antigens
EJMM-Egyptian Journal of Medical Microbiology [The]. 1995; 4 (1): 35-40
in English
| IMEMR
| ID: emr-37131
ABSTRACT
Serological diagnosis of 31 cases of extrapulmonary Mycobacteriosis [21 meningitis, 8 urinary, 10 bone and joint and one endometritis cases] 8 controls of other extrapulmonary cases and 16 healthy controls. ELISA test was used to detect serum Ig[G] and Ig[M] antibodies to 30, 000 Dalton [Da] and purified protein derivative [PPD] antigens of M. tuberculosis. The results were statistically analysed by accuracy index, sensitivity, specificity, positive and negative predictive values. Ig[G] detection using 30, 000 Da gave a higher sensitivity than PPD [66.6% and 52% respectively], while both antigens gave 100% specificity. On the other hand, the dual antigen gave a sensitivity of 85.7% and specificity 100%. A higher statistical significant difference was observed between cases and controls [P<0.005]. Detection of specific Ig[M] antibodies revealed no appreciable diagnostic value. It can be concluded from this study that the use of the dual antigen for detection of Ig[G] was reliable for the diagnosis of mycobacterial infection, however, it can't differentiate between Mycobacterial tuberculosis and other mycobacteria
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Tuberculosis
/
Tuberculosis, Meningeal
/
Tuberculosis, Osteoarticular
/
Tuberculosis, Urogenital
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
/
Immunologic Techniques
/
Mycobacterium tuberculosis
Limits:
Humans
Language:
English
Journal:
Egypt. J. Med. Microbiol.
Year:
1995
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