mycofast [MF] as a means of diagnosing and determining the incidence of urogenital mycoplasma implicated in persistent and/or recurrent nongonococcal uretheritis in men

ABSTRACT

The current work was designed to test the value of Mycofast [MF], a new repid method of urogenital mycoplasma [UGM] detection, in the diagnosis of Ureaplasma urealyticum and Mycoplasma hominis implicated in persistent or recurrent nongonococcal uretheritis in male patients. We aimed also at determinig the incidence of these pathogens in such clinical condition. Three samples either uretheral discharge or first voided urine were collected from each of 66 patients attending the Venereology Clinic, Armed Forces Hospital, Kuwait, between January- June, 1990 and suffering from persistent or recurrent NGU. The first sample was used for N. gonorrhea isolation, the second for UGM isolation by conventional methods and the third for inoculation of the MF kit. No N. gonorrhea was isolated. 26 samples grew U. urealyticum by conventional methods. Those 26 samples in addition to one conventional methods negative sample showed U. urealyticum picture on M. F after 24-72 hours. The number of growth ranged between 10[3]-10[6]CCU/ml. All strains except one were sensitive to cyclines and all except one were resistant to ciprofloxacin. M. hominis was detected neither by conventional method nor by MF. The incidence of U. urealyticum in the cases studied was 39% by conventional methods and 43% by MF. We conclude that MF is an ideal method for the diagnosis of UGM. Due to its high incidence, U. urealyticum should be considered in the empirical antibiotic regimen for patients with persistent or recurrent NGU