Identification of two different intracellular antigens in human leukocyte by monoclonal antibodies

ABSTRACT

Two monoclonal antibodies [mAbs] designated as SU11 and SUI2 were produced by fusion of a mouse myeloma cell line with spleen cells from mice immunized with human tonsillar lymphocytes. SUII and SUI2 monoclonal antibodies recognized two epitopes with molecular weights of 39 and 43 KDa derived from solubilized membrane of Nalm -16 and Bjab cell lines, respectively. Flow cytometric analysis revealed that these mAbs were reactive to around 30% of peripheral blood T cells and 25% of B cells and granulocytes, Nuclear and cytoplasmic staining was observed in 90% of bone marrow myeloid and lymphoid cells. These characteristic features of SUI1 and SUI2 mAbs suggest that the epitopes recognized by these mAbs are scattered throughout the cell membrane cytoplasm and nucleus