Efficacy of Elisa in diagnosis of strongyloidiasis among the immune compromised patients

ABSTRACT

Anti-strongyloides IgG antibodies were studied by conventional ELISA techniques among 115 immunocompromised and 36 patients parasitologically diagnosed to be infected by Strongyloides stercoralis through examining their GIT, and specimens. 36 individuals infected with other helminths and 30 healthy parasitologically free individuals were also included. The specificity of the technique using thawed and sonicated Strongyloides larvae as an antigen was 100%. The sensitivity was, respectively, 92.2% and 13.3% among the immune-competent and immune-compromised cases. When anti-human biotinylated IgG and avidin-perodixdase conjugates were used, sensitivity among both groups rose to 100% and specificity remained unchanged. Avidin-biotinylated ELISA was recommended for the diagnosis of hidden and disseminated strongyloidiasis