Detection of Epstein Barr Virus [EBV] in oral hemangioendothelioma [HE]

ABSTRACT

Oral HEs are exceedingly rare tumors. Six cases were included in the present study. All Patients were females and the tumors arise mostly from the gingiva, appeared as a large epulides; misdiagnosed as pyogenic granuloma or peripheral giant cell granuloma. No radiolucencies were observed except in three cases which recurred after surgical removal. These recurred three cases showed malignant criteria of pleomorphism, hyperchromatism and brisk mitotic figures more than the other three cases. All the patients were serologically positive to EBV, denoting previous infection. Biopsies were taken from all cases and stained for histochemical and histopathological examination. Immunohistochemical analysis was done using monoclonal antibodies for the demonstration of [1] Factor VIII related antigen,[2] Epstein -Barr virus [EBV]. Histological examination showed that three of the haemangioendotheliomas were of the epithelioid type, while the other three cases were of the spindle-cell type. All cases showed positive linear reaction to Gomori's stain surrounding the endothelidal cells and differentiating HE from haemangiopericytoma. The six cases showed the miniature luminae present intracytoplasmically by factore VIII R-AG, excluding epitheleoid haemangioma and squamous cell carcinoma. Expression of EBV in five cases out of the six immunoanalyzed cases, was detected for the first time in the literature