Effect of melatonin with vitamin E or verapamil on kainate-induced lipid peroxidation in rat brain

ABSTRACT

This work aimed to study the in vivo protective effect of melatonin [Mel] alone or in combination with either vitamin E [Vit E] or verapamil [Ver] against lipid peroxidation that induced by kainic acid [KA] in rat brain. KA was given in a single dose of either 5 mg/kg [LoKA] or 10 mg/kg [HiKA]. Mel [10 mg/kg] was injected three times every eight hours before KA administration. Vit E [200 mg/kg] or Ver [10 mg/kg] was injected once daily for seven days before KA. The protective effect of their combination with Mel was tested. The whole brain malondialdehyde [MDA] was assessed as an index of lipid peroxidation as well as the antioxidants; namely, glutathione peroxidase activity [GProx] and reduced glutathione [GSH]. It was shown that KA increased brain MDA content and GProx activity, while GSH level was decreased, and these effects were more pronounced with HiKA