Demethyltetracycline biosynthesis by streptomyces aureofaciens sub-species viridulaus as influenced by medium composition

ABSTRACT

The influence of different nutritional compounds on demethyltetracycline by Streptomyces aureofaciens subspecies viridulaus was examined. Of the variety of carbon sources, nitrogen and phosphorus compounds tested; fructose, sodium nitrate and sodium dihydrogen phosphate supported the highest demethyltetracycline production. The addition of ferrous sulfate and nicotinamide [as trace element and vitamin, respectively] stimulated the biosynthesis of the antibiotic. In the majority of cases, the biomass yield ran parallel with the antibiotic production