Changes in serum proteins, lipids and some enzymes due to chelating agent [EDTA] treatment in lead intoxication

ABSTRACT

The effect of chelating agent EDTA on some biochemical parameters has been studied on lead exposed rats that were fed on semi-purified diet and were receiving 100 micro g/ml lead as acetate in drinking water. After 4 months, the lead exposed rats were intraperitoneally injected with EDTA for five successive days. The results indicated that ALA-D activity was inhibited by lead intoxication and EDTA treatment did not induce any improvement of the inhibited activity. On the other hand, ZPP increased significantly by lead exposure but tended to decrease after EDTA administration though it is still significantly higher than controls. EDTA treatment induced a significant decrease in total proteins of lead exposed animals which is due to the significant decrease in albumin and gamma -globulin fractions. While lead exposed animals did not show any change in cholesterol level, EDTA administration caused a significant hypocholesterolemia. EDTA treatment was found to induce significant increase in ceruloplasmin and inhibition in SGOT. It seems that EDTA treatment is followed by some biological changes that could be attributed to the chelating effects of EDTA to some trace metals