Detection of trichinella pseudospiralis antigen in the parenteral phase in experimentally infected mice

ABSTRACT

Antigen distribution of T. pseudospiralis in muscles was studied at 10 days and 2, 3 and 5 weeks post-infection. Immunocytolocalization of antigen in the muscle was performed by peroxidase antiperoxidase [PAP] and immunogold silver staining techniques. Weak positive reaction of the stichosome and the cuticle was observed at 10 days post- infection which increased to attain its highest level in the fully infective stage larvae, 3 and 5 weeks post-infection. The intensity of staining reaction in the cytoplasm and nucleoplasm of infected host cell and surrounding tissue was the highest at 5 weeks post- infection. The small nuclei of the host cells and the inflammatory cells were unstained