Rapid detection and titration of rinderpest antibodies in bovine sera using the chamber immunofluorescent technique

ABSTRACT

Chamber immunofluorescent technique [CIT] was compared with the serum neutralization [SNT] and enzyme-linked immunosorbent assay [ELISA] for the detection of rinderpest virus antibodies in bovine sera. ELISA was found to be the most sensitive test, while CIT compared with SNT was more specific than ELISA. Also, results of CIT were obtained in less than 2 hours. Moreover, titers detected by CIT were comparable to those obtained by SNT. In conclusion, CIT was found to be a reliable, rapid, specific and sensitive technique for the detection of rinderpest antibodies in bovine sera