Studies on haemophilus species in raw milk

ABSTRACT

A total of 100 raw milk samples were collected from buffalo farms and retail stores [50 samples each] for the detection of Hemophilus species, 20% of the examined samples were contaminated with hemophilia, 11 samples [22%] from buffalo farms and 9 [18%] from retail stores. The isolated species were H. influenzae [6%], H. parainfluenzae [5%], H. segnis [4%], H. aphrophilus [3%] and H. parphrophilus [2%]. Heat treatment of milk either by pasteurization or boiling was sufficient to inactivate all the isolates of hemophilia. Addition of different antibiotics as a supplement to the growth medium resulted in the isolation of hemophilia with different rates. Public health importance of recovered hemophilia has also been discussed