Isolation and preparation of bovine rota virus [BRV] vaccine in minnesota, USA

ABSTRACT

Nine cytopathic bovine rota virus strains were isolated in MA104 cells from fecal specimens of calves. Plaque assay was used for selection, titration and purification of small and large plaques of selected rota virus strains. Electron microscopy was used to detect the presence of particles with rota virus morphology in MA104 cells. Virus neutralization test was used for further identification of strains of high immunogenic response. Pooling of these isolated and their use in making a formalized-inactivated bovine rota vaccine was tried