Volatile oil produced by microbial infected Rosmarinus officinalis callus and suspension cultures

ABSTRACT

Leaf fragments of Rosmarinus officinalis were grown in a media supplemented with indoleacetic [IAA] or 2, 4-dichlorophenoxyacetic acid [2, 4-D], in both the presence and absence of benzyladenine [BA]. The leaf fragments showed callus growth after the third week of culturing. A high level of BA was correlated to callus browing. Culturing of rosemary on media supplemented with 2, 4-D enhanced callus formation. Callus from rosemary were transferred to Murashige and Skoog [MS] liquid medium supplemented with 2, 4-D, and BA. Cineole and beta- pinene were identified using TLC. Both callus and cell suspension were found to be infected with a single type of bacteria strain, which was identified as Pseudomonas mallei. Growth inhibition of the bacteria was examined using 10 different antibiotics. Chloramphenicol, Cefoxitin, Ceftriaxone and Tazocin showed pronounced inhibitory effects, while the other six antibiotics exhibited little or no inhibitory effects