Application of polymerase chain reaction to detect rearrangement of immunoglobulin heavy chain genes in lymphoproliferative disease
Annals of Saudi Medicine. 1997; 17 (4): 395-8
in English
| IMEMR
| ID: emr-43949
ABSTRACT
As part of our routine work-up in the diagnosis of lymphoproliferative disease, we used a rapid polymerase chain reaction [PCR] assay to amplify the DNA fragments of the framework 3 [FR3] region of the immunoglobulin heavy [IgH] chain genes. The assay does not involve hybridization, nested priming, or sequencing of the amplified PCR product. It was performed on 66 specimens of B-cell lymphoproliferative disease, including acute lymphoblastic leukemia, chronic lymphocytic leukemia, multiple myeloma, hairy cell leukemia and follicular lymphoma. Twenty-six specimens of negative controls, including acute myeloid leukemia, chronic myeloid leukemia in myeloid transformation and idiopathic thrombocytopenic purpura, were also analyzed. The assay was performed with 77% sensitivity and 100% specificity. The standard IgH chain gene rearrangement by Southern blot analysis is reserved for the remaining negative cases if clinically indicated
Search on Google
Index:
IMEMR (Eastern Mediterranean)
Main subject:
Genes, Immunoglobulin
/
Gene Rearrangement
/
Polymerase Chain Reaction
/
Immunoglobulin Heavy Chains
Language:
English
Journal:
Ann. Saudi Med.
Year:
1997
Similar
MEDLINE
...
LILACS
LIS