SDS-page of mononuucl cell protein lysates of HLA - identical

ABSTRACT

The loci responsible for the most vigorous graft-rejection reactions are contained within the HLA complex in humans. However, even when a donor and recipient have identical HLA antigens, differences in minor histocompatibility loci outside the major histocompatibility complex [MHC] can also contribute to allograft rejection- This study dealt with the detection of differences other than HLA, between individuals, that may account for rejection of grafts. Mononuclear cell protein lysates were prepared following two different methods. Lysates from a pair of HLA-identical twins and from 10 HLA non-identical individuals were run on 12.5% reducing Sodium dodecyl sulphate SDS Polyacrylamide Gels. All Polyacrylamide Gels were silver stained. Mononuclear cell protein profiles were determined on the basis of the number of bands, their molecular weights and band intensity. HLA identical Individuals had the same mononuclear cell protein profiles. Slight differences in protein profiles were noted, however, between HLA non identical individuals. Observed differences were mainly confined to band intensities, which may indicate that some proteins may be over expressed in certain individuals. This over- expression may have important implications in graft rejection