Effect of serum and protein synthesis inhibitors on polymorphonuclear neutrophil apoptosis
Medical Journal of the Islamic Republic of Iran. 1997; 10 (4): 285-9
in English
| IMEMR
| ID: emr-45590
ABSTRACT
In this study the programmed cell death [apoptosis] of human neutrophilic granulocytes was investigated in the presence and absence of fetal calf serum [FCS], cycloheximide and actinomycin-D. The results show that when FCS is omitted from cultures, apart from a decrease in viability, the percentage of apoptotic cells and DNA fragmentation increases. Apoptosis is accelerated in serum withdrawal cultures at 6 hours of incubation time. The use of fluorescent dyes and diphenylamine reaction procedures confirm the above results. Treatment of cells with protein synthesis inhibitors, actinomycin-D and cycloheximide promotes apoptosis and produces a typical ladder of internucleosomal cleavage in the cellular chromatin; the extent of fragmentation however, differs
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Protein Synthesis Inhibitors
/
Cycloheximide
/
Dactinomycin
/
Neutrophils
Limits:
Humans
Language:
English
Journal:
Med. J. Islamic Rep. Iran
Year:
1997
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