Hyaluronic acid mediated adhesion of pasteurella multocid a to different host cells

ABSTRACT

The in vitro adhesion of 12 Pasteurella multocida [isolated from different sources] was studied on turbinate and tracheal epithelial cells from porcine and bovine as well as on HeLa-S3 cells. All serotype A isolates [8 from rabbits and one from human] adhered to all cell models with distinctively higher affinity to HeLa cells than turbinate or tracheal cells. Serotype B isolate from bovine [one isolate] failed to adhere on any cell model, whereas serotype D from rabbit [one isolate] adhered weakly to all cell models. The same serotype [D] from buffalo [one isolate] adhered weakly to HeLa cells and bovine cells and failed to adhere to the porcine cells. Altering the P. multocida outer surface characteristics using specific antiserum or protein denaturing agents as heat and acidity [95C 1 hour and pH 5], trypsin [250 units/ml], pronase-E [100 ug/ml] did not affect the bacterial ability to adhere to host cells. Only hyaluronidase treatment [50 units/ml] reduced the adhesion drastically. On the other h and, blocking the host cell receptors with N-acetyl glucosamine or D-glucuronic acid [hyaluronic acid monomeres] did not inhibit the adhesion. Only locking the HeLa cells with hyaluronic acid [10 mg/ml] reduced or stopped the ability of P. multocida to adhere to the HeLa cells