effect of idoxuridine treatment on the antibody level in herpetic stomatitis cases, using the enzyme linked immunosorbent assay [ELISA]

ABSTRACT

Sixty four children clinically diagnosed as having herpetic gingivo stomatitis were included in the study. Scrapings from the lesions were inoculated on vero cell tissue culture cells for HSV isolation. Acute and convalescent sera were collected from all patients as well as from 50 control children. 45 HSV isolates were obtained. Half of these cases received idoxyuridine local ointment and the other half had 1% gentian violet paint. Antibodies were tested by the enzyme linked immunosorbent among [ELISA] using goat horse radish peroxidase cenjugate antihuman IgG as a substrate optical density was measured by Dynatech microplate ELISA reader at 410 nm filter. Significant difference was detected between the acute convalescent HSV antibody titer. The difference between HSV antibody titers in those received IDU and gentian violet was found to be statistically non significant. It was concluded that IDU acts only locally and has no remote or systemic effect on the virus or the antibody production capacity