Vibrio cholerae 0139 outbreak in sibicity, balochistan

To isolate the etiological agent of severe diarrheal outbreak, identification and antibiogram of isolated agent. Design: Samples of stool and water supply of affected area collected in Alkaline peptone water inoculated on special media. The isolates strain was tested against 8 antibiotics. Isolated strain and antibiogram confirmed by NIH Islamabad. Setting: Specimen collected from Sibi City near Quetta where outbreak of severe diarrheal disease reported. Specimen was tested at Sandeman [Prov.] Hospital, Quetta. Prospective study involving four patients suffering from severe diarrheal disease admitted in Civil Hospital Sibi. Two specimen of water collected from different parts of affected area. Main outcome measures: Isolation of V. cholerae 0139 and antibiogram against isolated strain. The etiological agent responsible for severe diarrheal disease outbreak in which 110 cases and 4 deaths [3.63%] reported was vibrio cholerae 0139. The strain was the same in all patients and water supply. Susceptibility to antimicrobials was determined showing sensitive to Chloramphenicol, Erythromycin, Nalidixic Acid, Norfloxacin and Tetracycline, resistant to Polymyxin B, Co-trimoxazol, and intermediate to Ampicillin. V. cholera 0139 isolated as etiological agent for severe diarrheal disease outbreak, which was spread through drinking, water. The new strain has potential for severe outbreak like classical type. New strategies are needed to control the outbreaks of the new strain