Lymphocyte transformation in nephrotic patients

ABSTRACT

Lymphocyte transformation has been shown to be inhibited by sera of nephrotic patients in vitro. The inhibition of lymphocyte transformation by nephrotic sera was attributed to hypoalbuminemia or hyperlipoproteinemia. In the present work we studied the effect of sera of patients suffering from nephrotic syndrome on blastoid transformation in vitro. The role of hypoabuminemia and hyperlipoproteinemia was investigated. Ten cases of nephrotic syndrome and ten normal controls were included. They were subjected to complete blood picture, complete urine analysis, blood urea, serum creatinine, 24-hours urinary protein excretion, total serum proteins and serum proteins electrophoresis, total serum lipids, serum triglycerides, total serum cholesterol and high density lipoprotein cholesterol electrophoresis, in addition to lymphocyte studies which included separation, culture and blastoid transformation of patient's lymphocyte in the presence of the mitogen [phytohemagglutinin] and the effect of nephrotic sera on this process. Blastoid transformation was studied in the presence of whole patient's serum, patient's after albumin substitution to 4.5 gm/dl; serum of the patient after separation and removal of lipoprotens; serum of the patients after lipoprotein separation and albumin substitution