Comparative studies between local and imported combined inactivated vaccines containing BVD, IBR and PI-3 viruses

ABSTRACT

Comparative studies to evaluate the immunogenic of the local inactivated combined vaccine incorporating IBR,PI-3 and BVD viruses prepared from the local isolates growth in MDBK cell line and inactivated by binary ethyleneimine and adsorbed with 20%. Alhydrogel adjuvant, and the imported inactivated vaccine which contains PI-3, IBR and BVD-MD and respiratory syncytia vaccine [Triangle 4]. The kinetics of immune response are elicited by ELISA Immuno Assay [EIA] and serum neutralization test [SNT] against the same component of the vaccine. The protective capacity of each vaccine was studied by challenge exposure which was performed at one month post vaccination [PV] assured that vaccinated animals resisted infection well. The non vaccinated control infected animal showed a high rate of thermal reaction and viraemia. The results of virus isolation could be confirmed by fluorescent antibody technique. The present data explained a significant higher degree of immunogenicity demonstrated with the local prepared vaccine than the imported against all the same categories of the two vaccines