Fluorometric determination of amiodarone. amlodipine and propafenone

ABSTRACT

Three fluorimetric methods have been developed for the determination of some cardiovascular drugs. Amiodarone hydrochloride in 0.1 N sulphuric acid showed native fluorescence with excitation at 270 nm and emission at 540 nm. Methanolic solution of amlodipine besylate exhibits maximum excitation and emission at 365 nm and 450 nm, respectively. Propafenone hydrochloride forms fluorescent ion-pair with eosine at pH 5, which is extractable in chloroform, the excitation and emission at 318 nm and 550 nm, respectively. Linearity is obtained upon determining authentic samples in concentration range of 40 - 200 mug/ml, 0.4-2.8 mug/ml and 7-24.5 mug/ml for amiodarone, amlodipine and propafenone, respectively. The precision of the proposed method is checked by analyzing different samples of bulk powder and mean percentage recoveries are 100.26 +/- 1, 99.83 +/- 1.03 and 100.2 +/- 0.91 for amiodarone, amlodipine and propafenone, respectively. The pharmaceutical formulations are also estimated applying the proposed fluorimetric methods using st and ard addition technique, showing accurate results having great agreement with those of the reference methods: