Effect of some physiological changes on the binding of tenoxicam to human serum albumin

ABSTRACT

The influence of some physiological changes due to age or disease on the binding of tenoxicam to human serum albumin [HSA] was studied. Increasing HSA concentration within the range of 0.04% to 0.4% decreased the binding parameters, K1, n1 and n2. The presence of fatty acids decreased the affinity of HSA binding to tenoxicam at low protein concentrations. Urea as well as glucose increased the binding affinity of HSA to tenoxicam. The values of n1 and n2 were not significantly changed except when glucose was elevated to 500 mg/dL causing n1 and n2 to be nearly halved. Binding of tenoxicam to HSA increased as pH increased within the tested values of 6.8, 7.4 and 8. Consequently, tenoxicam was preferably bound by B rather than N form of HSA