Rapid detection of IgM and IgG antibodies to herpesviridae virus. by colorzyme EA test in comparison with CFT, IF and ELISA tests

ABSTRACT

In this study, the Immunoconcepts EA indirect enzyme antibody technique [colorzyme] was used not only for detection of IgG antibodies but also for quantitative detection of IgM antibodies to Herpes Simplex Virus [HSV], Cytomegalovirus [CMV] and Epstein Barr Virus [EBV] to diagnose recent active infection. Reference reactive and negative antisera and randomly collected human sera were tested by complement fixation test [CFT] against HSV antigens and tested also by immunofluorescent [IF] and colorzyme Immunoconcepts EA tests. All sera that were negative to HSV, CMV and EBV antibodies by CFT were negative by IF and colorzyme EA tests. All antibody positive sera and reference positive antisera were also positive by IF and colorzyme EA tests with slight variation in antibody titres between CFT and colorzyme test results. Human sera which were negative or IgM positive to HSV, CMV and EBV by ELISA as well as negative and positive reference sera from different diagnostic kits were retested by IF and colorzyme EA for IgM. Antiviral reactivity results were concordant by the three tests. All incubations in colorzyme test were at room temperature and only an ordinary microscope used in IF test or plate washers and readers needed for ELISA test. The colorzyme immunoconcepts is a simple, rapid and sensitive technical for viral diagnosis and can be used in any private laboratory