PCR-mediated expression of the human GM-CSF gene in escherichia coli
IBJ-Iranian Biomedical Journal. 1998; 2 (1): 21-25
in English
| IMEMR
| ID: emr-48108
ABSTRACT
Four exons of the human genomic GM-CSF gene were assembled together using gene splicing by overlap extension [SOE] method. The resulting nucleotide sequence was cloned in the pET23a [+] expression vector under the control of strong bacteriophage T7 transcription and translation signals. The construct obtained was Transferred into the E. coli strain, BL21 [DE3] pLysS and IPTG was used for induction of GM-CSF gene and production of the target protein, one mg of protein per liter of cell culture, was obtained as revealed by ELISA
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Gene Expression
/
Polymerase Chain Reaction
/
Escherichia coli
Language:
English
Journal:
Iran. Biomed. J.
Year:
1998
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