Hepatitis B and C virus infections among patients with gingivitis and adult periodontitis: seroprevalence and public health importance

ABSTRACT

The aim of the present case control study was to identify seroprevalence of hepatitis B and C virus [HBV and HCV] infections among one hundred cases with periodontal disease [71% gingivitis and 29% adult periodontitis] and one hundred controls with healthy gingiva matched for age and sex. Moreover, it aimed to detect hepatitis markers in saliva samples corresponding to the positive sera. Different risk factors associated with hepatitis infection and detectability rate of hepatitis markers were also studied. Methodology: enzyme linked immunosorbent assay [ELISA] technique was used to detect the presence of hepatitis B surface antigen [HBsAg], antibody to hepatitis B core antigen [anti HBc], e antigen [eAg] and antibody to hepatitis C virus [anti HCV] both in serum and saliva samples. HCV RNA was detected by polymerase chain reaction [PCR] technique. Oral examination was performed for assessment of simplified Oral Hygiene Index [OHI-S], Papillary Bleeding Index [PBI], probing pocket depth and loss of fibre attachment. cases with periodontal disease showed higher percentages of hepatitis exposure [hepex; anti HCV and/or anti HBc] and significantly higher anti HCV seropositivity than the controls [26% and 13% versus 22% and 8%, respectively]. No difference in HBsAg carrier rate nor in anti HBc seropositivity was elicited. Furthermore, cases with periodontal disease showed higher detectability rate of HBsAg, anti HBc, anti HCV or both anti HCV and/or anti HBc in whole unstimulated saliva than the controls [100% vs 66.7%, 50% vs 23.5%, 23.1% vs 0.0% and 42.3% vs 18.2%, respectively]. Stepwise logistic regression delineated two significant factors associated with the risk of hepatitis exposure, the first predictor was the rural residence and the second one was the history of blood transfusion [OR=3.10, 2.94, respectively]. Periodontal disease, severity of bleeding and bad oral hygiene were associated with the risk of hepatitis infection and with the detectability of hepatitis markers in the whole saliva