Histological changes of the remaining live donor's kidney in rat. A light microscopic, electron microscopic and morphometric study

ABSTRACT

Twenty adult male albino rats were used in this study. The results revealed a significant increase in all the parameters measured in the remaining kidney after 2 months. Their weight was increased by 160%, the glomerular surface area increased by 140%. The proximal tubules showed an increase of their diameter by 27% and the height of their lining cells increased by 30%. With PAS technique, focal and diffuse thickenings of glomerular basement membrane were evident together with increased mesangium. However, hydropic degeneration and tubular necrosis were detected in many tubular cells after 2 months. With PAS, their basement membranes showed focal thickenings and their brush borders were thin and interrupted. The electronmicroscopic examination of the renal cortex after one month displayed focal thickenings in the glomerular basement membranes mainly at lamina densa. Marked narrowing of the glomerular capillary lumina by the proliferating endothelium and mesangium was observed. Mitochondria were swollen, vacuolated and lost their parallel alignment, endoplasmic reticulum appeared swollen. Lysosmes and pinocytotic vesicles were increased