Potential cytogenetic effect of Lupinus terms seeds extract

ABSTRACT

The cytogenetic effect of 70% ethanolic extract of Lupinus termis seeds was investigated in mouse bone-marrow, spleen and spermatocyte cells. Mice were orally treated by gavage with a single dose of 1.05, 2.10 and 4.20 g/kg b.wt. which correspond to [1/8, 1/4 and 1/2 of LD50]. For bone marrow and spermatocyte cells daily successive dose treatment [1.05] g/kg b.wt for 3, 7 and 10 days were performed, the same dose was given daily for only 5 successive days for spleen test. "Mitomycin C" at a dose of I mg/kg b.wt was used as a positive control. Results reveal that Lupinus termis extract caused slight increase in the frequency of chromosomal aberrations in bone-marrow and spleen cells. Such increase was found to be non-significant at all treatment levels [single and repeated] after excluding gaps. With respect to mouse spermatocytes the extract had no significant effect. Comparing the chromosomal aberration effect of Lupinus termis extract with that obtained from treatment with "Mitomycin C", results generally indicate that the 70% ethanolic extract of Lupinus termis had no remarkable cytogenetic effect in both somatic and germ cells