Interference of anticoagulants with estimation of glucose alkaline phosphatase, albumium, O-analyse and total protein

ABSTRACT

The effect of anticoagulants, [EDTA, lithium heparin and Na-citrate] on the estimation of some clinical test [Glucose, Alkaline phosphatase, Albumin, Total protein and alpha-Amylase] was investigated. The interference of these anticoagulants seems to be very high. Na-citrate plasma was found unsuitable specimen for alkaline phosphatase and Glucose estimation. Lithium heparin plasma overestimated Albumin level Despite that alpha-amylase concentration was not affected by the anticoagulants, it was found out side the reference range recommended by the procedure of determination. It was confirmed that EDTA plasma is preferred over serum as specimen for total protein estimation. This study was conducted on thirty fasting volunteers [n=30]. Control was run as a replicate specimen to validate the results obtained. To avoid unexpected analytical artifacts, serum is recommended as suitable specimen for the determination of most of clinical tests