use of different media in the isolation and maintenance of campylobacter jeiuni from children with diarrhea

ABSTRACT

Among a total of [105] samples collected from childern suffering from bloody or mucoid diarrhea, 11 [10.5%] isolates were diagnosed as Campylobacter [C.] jejuni, of which 2 [82%] were identified as Biotype 1, and 2 [18%] as Biotype 2, In the primary isolation procedures, Campylobacter blood-free selective medium [CBFM] was superior to skirrow's [SM] and Preston's [PM] media. Regarding the enrichment maintenance and viability of campylobacters, a newly prepared medium, the Columbia Biphasic medium [CBM] was superior to Brucella broth [BB], nutrient broth No. 2 [NB2] and fluid Thioglycollate medium [FTM]