Celliulolytic activity of thermomonospora fusca: II-determination of optimal conditions for extracellular endoglucanase, exoglucanase and beta-glucosidase activity in the selected isolate TA[19]

ABSTRACT

Extracellular cellulase crude enzyme complex preparation was obtaind. from the culture filtrate of Thermomonospora fusca isolate [TA[19]], grown in kosmachev liquid medium for [9] days at pH [9] and 45°C.Enzyme assays were carried out to determine the influence of time, temperature and pH required for maximal enzymatic activities of endoglucanase,exoglucanase and beta-glucosidase. Results showed that, higher enzyme activity was after [10,20,10] min at temperature [65,65,55°C] and pH [6] for mentioned enzymes, respectively