DNA analysis of hepatocytes: a novel method for estimating the value of liver tissue vaccine [LTV]

ABSTRACT

Because of the socio-economic condition of African countries, the traditional types of vaccines should be encouraged. LTV in a preliminary study proved to be of value in reducing morbidity and mortality in Schistosoma mansoni infection. In the present study, and because of the vaccine contains liver tissue homogenate, the present study analyzed the DNA content of the hepatocytes of the vaccinated and control groups of animals to study any effects of the vaccine on the hepatocytes ploidy. The DNA content of vaccinated animals was high. The results were discussed. It was concluded that for testing S. mansoni vaccine, DNA analysis of hepatocytes for detecting proliferative S-phase is an important as a positive immune reaction