Effect of taxol on the expression of transforming growth factor beta-1 in ehrlich ascites carcinoma cells

ABSTRACT

Ehrlich ascites carcinoma cells [EAC] were found relatively sensitive to taxol and exhibited a significant apoptotic response following treatment in vitro. The effect of taxol treatment on the expression of transforming growth factor beta one TGF- [Beta1] in vitro was examined. EAC cell line [6 x 10[6] cell] were exposed to different concentrations of taxol for different intervals of time, and the amount of intracellular TGF-[Beta1] was measured using ELISA technique and western blotting. Results revealed that 50 nM of taxol is the optimum concentration at which TGF-[Beta1]. was expressed. Also TGF-[Beta1] 1 expression was maximum after 24 hours of exposure to taxol rather than the other exposure times [2,4,6 h]. The mechanism of tumor responsiveness to taxol associated with increased expression of TGF-[Beta1] is discussed