Cloning and expression of acd genes of FMDV
Veterinary Medical Journal. 1999; 47 (3): 289-294
in English
| IMEMR
| ID: emr-53052
ABSTRACT
RT-PCR amplification of VIA [3D] gene and protease [3C] using its primers. Legation of the PCR product with the cloning vector [using pET system]. Expression of the recombinant plasmid in E. coli expression host. Use of the expressed VIA protein in diagnosis of cattle infected with any of the 7 serotypes of FMD virus with the help of ELISA
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Cattle
/
Enzyme-Linked Immunosorbent Assay
/
Aphthovirus
/
Cloning, Organism
Limits:
Animals
Language:
English
Journal:
Vet. Med. J.
Year:
1999
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