correlation between gingival inflammation, gingival fluid, pH and types of microorganisms in dental plaque

In physiological and pathological conditions, the gingival fluid pH play a significant role on the structure and function of the gingival crevice. The aim of the study was to evaluate the clinical correlation between gingival fluid pH, Gingival status and microbial shift during the establishment of gingivitis experimental gingivitis carried on 7 dental [mean age was 21 Yr.]. Gingival fluid pH evaluated by using universal indicator paper. The salivary pH and blood pH was also evaluated from the same patients by using the same method. Supragingival plaque was examined by dark field microscope. The results showed that the mean of the gingival fluid pH was 6.5 in patients with healthy gingiva [GI=0]. While with the development of experimental gingivitis [GI=1]. The mean gingival fluid pH was 6.8. Gingival fluid pH also evaluated after the resolution of gingival inflammation [GI=0]. The mean pH was returned again to 6.5. Bacteriological examination of the supragingival flora of day zero consisted mainly of cocci and rods. At day 7, the group of cocci dropped markedly. Fusiform filaments and motile increased slightly in their proportion. At day when gingivitis developed. In addition to the type of bacteria already found at day 7. Spirochetes also found, no statistical difference was shown in all time intervals of the experiment for both bacteriological and gingival fluid pH. The results also demonstrated a stable pH in both saliva and blood during the period of the experimental gingivitis. In conclusion we can say that there is a correlation between the gingivitis and gingival fluid pH value and this may be due to increase in the vascular permeability which considered as one of the important feature of the gingival inflammatory response