protective effect of dimethyl-4,4' dimethoxy - 5,6,5',6' - dimethylenedioxybiphenyl -2.2' - dicarboxylate [DDB] on isolated rat hepatocytes compared with vitamin E

ABSTRACT

This work aimed to compare the protective effect of dimethyl- 4, 4'dimethoxy-5, 6, 5', 6'-dimethylenedioxybiphenyl-2, 2'dicarboxylate [DDB] with that of vitamin E against the hepatotoxicity induced by carbon tetrachloride on isolated rat hepatocytes. Vitamin E and various concentrations of DDB were preincubated in the isolated rat hepatocyte suspension for 30 minutes before being subjected to the hepatotoxin CC14 for an additional 150 minutes. Lactate dehydrogenase [LDH], malondialdehyde [MDA] for lipoperoxidation and the depletion of reduced glutathione [GSH] were measured as indices for liver damage. Protection against the induced cell injury was conferred by DDB as evidenced by the decreased leakage of LDH, the inhibition of lipoperoxidation and the restoration of cellular glutathione. Vitamin E induced similar hepatoprotection against CC14-induced damage, but to a lesser extent than DDB