PJMR-Pakistan Journal of Medical Research. 2000; 39 (4): 135-138
in English
| IMEMR
| ID: emr-55077
ABSTRACT
Semen analysis is considered to be the single most useful procedure of assessing subfertility. Thus, the level of fructose, antibodies, Zinc and reproductive hormones have been studied in different types of subfertile males. The level of N-acetyl[3-D glucosominidase [NAG] activity, an enzyme in the aerosomal cap, has also been studied but its levels have not been compared with asthenospermic and necrospermic males. The current study was conducted to evaluate the role of semen NAG activity and plasma reproductive hormone [LH, FSH and Testosterone] that correlate with the different types of subfertility in males. Thirty eight normal healthy, 24 oligospermic, 14 azoospermic, 5 asthenospermic and 2 necrospermic males between 24-45 years of age with no physical or endocrinal disorder were included in the study. Semen samples were collected with the standard procedure in sterile glass tubes and sperm count was measured by using Horwell Fertility Counting Chember. The NAG activity in seminal plasma was estimated by the fluoremetic method. Semen protein content was determined by Lowry's method. Plasma LH, FSH and testosterone in all subtypes of sub fertile men were determined by radioimmunoassay method. NAG activity in normospermics was found to be 0.349'0.0031 ug/mg protein, whereas, in oligospermic and in azoospermic males, the activity significantly [P<0.05] raised as compared to values in normospermic males. Plasma Testosterone concentration was found significantly lower in oligospermic and higher in asthenospermic whilst there was no difference between azoospermic and normosperic count. Whereas, plasma LH and FSH were significantly elevated in oligospermic and azoospermic groups. The current data indicate a direct relationship between plasma testosterone level and semen NAG activity and an inverse relationship with plasma LH/FSH. Semen NAG activity in males could be used to derive a male fertility prediction value in selected couples with no identifiable female factor of infertility
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Oligospermia
/
Reproduction
/
Semen
/
Testosterone
/
Luteinizing Hormone
/
Follicle Stimulating Hormone
/
Hormones
/
Infertility, Male
Limits:
Humans
/
Male
Language:
English
Journal:
Pak. J. Med. Res.
Year:
2000
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