Indirect determination of glucose by atomic absorption spectrophotometry

ABSTRACT

Although atomic absorption spectrophotometry is commonly used for the direct determination of metals, the method is not suitable for the determination of organic substances. Various methods for the indirect determination of organic compounds are however used frequently 2-Hydroxynaphthoic acid has been determined indirectly through the selective extraction of the Ni [II] 2-Hydroxynaphthoate with nitrobenzene. Phthalic acid is extractable into methyl-isobutyl ketone as the ion pair formed between bis-[neocuproin] Cu [I] and the univalent anion of phthalic acid. A similar method has been employed for the determination of pentachlorophenol. Halonen and Aira were able to determine sugars by their depressive effect on the absorption of calcium. Sugar was also determined by atomic absorption Spectrophotometry after the reduction of CuSO[4] in a carbonate-tartarate solution. Berka et al. studied the oxidation of D-glucose by KMn0[4] in buffered and alkaline media. In the present investigation, and indirect method for the atomic absorption determination of glucose is developed based on the reaction with KMnO[4] under such conditions leading to the quantitative formation of MnO[2]. Both the unreacted KMnO[4] and formed MnO[2] are then determined by atomic absorption Spectrophotometry. Thus, the atomic absorption method for the determination of Mn is indirectly used for the determination of glucose