antigenic fractions of strongyloides stercoralis filariform larval crude soluble antigen

ABSTRACT

This study was carried out on the sera of fifteen Strongyloidiasis patients, twenty-five cases with other helminthic infections and five cases free from parasitic infections [control]. Enzyme immunoblot transfer assay [EIBT] was used to assess IgG response against the different fractions of Strongyloides stercoralis filariform larval crude soluble antigen separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis [SDS-PAGE]. In the immunoblot, all sera of 15 strongyloidiasis patients disclosed IgG antibodies reactivity against immunogenic bands having molecular weights. Five immunodominant antigenic bands of 66, 50, 41, 38 and 31 KDa were commonly recognized by the strongyloidiasis sera IgG suggesting that these antigenic components may be suitable for specific immunodiagnosis of human strongyloidiasis